Additional file 4: Movie 3.


3D reconstruction of an uninfected MDM expressing PH-GFP. Uninfected MDMs were nucleofected to express PH-GFP for 24 hours, fixed and imaged by confocal microscopy. Fiji software was used to assemble a 3D reconstruction from 230 optical z-slices (step size of 0.04 μm). Cell as shown in Figure 1I-K.

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Mlcochova et al. BMC Biology 2013 11:89   doi:10.1186/1741-7007-11-89