Additional file 3: Movie 2.
3D reconstruction of an uninfected MDM labeled with CellMask. Uninfected MDMs were labeled with CellMask for 5 minutes at 37°C. Confocal sections were recorded using an UltraVIEW Vox spinning disc confocal system (PerkinElmer, Cambridge, UK). Fiji software was used to build this 3D reconstruction assembled from 165 optical z-slices (step size of 0.1 μm). Cell as shown in Figure 1D-F.
Format: MOV Size: 9.2MB Download file
Mlcochova et al. BMC Biology 2013 11:89 doi:10.1186/1741-7007-11-89