Additional file 2: Figure S1.

Cell-to-cell variability in the size and morphology of the IPMCs. (A) MDMs were nucleofected to express Gag-GFP and stained with FM 4-64FX 24 hours later. Confocal sections show co-localization of Gag-GFP and the FM 4–64 dye in the IPMC. (B) MDMs were labeled with the membrane-impermeable CellMask for 5 minutes at 37°C (or 30 minutes at 4°C; bottom row in panel B) and fixed. Confocal sections show labeled cell surface and IPMCs in uninfected and HIV-infected MDMs from the same donor. (C) MDMs were nucleofected with PH-GFP. Confocal sections show labeled cell surface and IPMCs in uninfected and HIV-infected MDMs from the same donor. Infected MDMs were detected by staining for the HIV matrix protein p17 (bottom panels). All scale bars: 10 μm.

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Mlcochova et al. BMC Biology 2013 11:89   doi:10.1186/1741-7007-11-89