Table 3

Antibodies used in this study
Antibody Company Reference number Dilution
Primary
EpCam AbCys abc171 1:20
CK19 Dakoa M0888 1:50
AFP Dako N1501 1:300
HNF4α Santa Cruzb SC-8987 1:100
E-cadherin Dako M3612 1:100
SOX17 R&D MAB1924 1:100
OCT4 Santa Cruz SC-5279 1:200
NANOG R&D AF1997 1:100
SSEA 4 AbCys VMA 4304 1:100
TRA 1-60 Millipore MAB4360 1:100
Secondary
Goat anti-mouse Molecular Probesc A21123 1:800
Chicken anti-goat Invitrogen A21467 1:800
Goat anti-rabbit Molecular Probes A11010 1:800

aDako, Glostrup, Denmark.

bSanta Cruz Biotechnology Inc., Santa Cruz, CA, USA.

cMolecular Probes, Eugene, OR, USA.

Yang et al.

Yang et al. BMC Biology 2013 11:86   doi:10.1186/1741-7007-11-86

Open Data