Specificity of apolipoprotein A-II (APOA-II) promoter for hepatic cells. (A) Schematic diagram of the APOA-II-green fluorescent protein (APOA-II-GFP) lentivector. (B) Relative mean fluorescence intensity (MFI) representing GFP expression was evaluated in various types of cells: different epithelial cell lines (A549, HeLa, MCF7), hepatoma cells (HuH7), primary fibroblasts, human umbilical vein endothelial cells (HUVECs), and human pancreatic (COP) cells. Non-transduced control (NT, grey bars), transduced with elongation factor (EF)1α lentivector (black bars), or with APO-AII-GFP (white bars) lentivector. (C) Fluorescence-activated cell sorting (FACS) analysis of GFP-expressing fibroblasts 3 days after transduction with APOA-II-enhanced (e)GFP or EF1α-eGFP lentivectors. (D) Phase-contrast and fluorescence micrographs after the transduction of mesenchymal stem cells (MSCs) with EF1α-GFP and APOA-II-GFP lentivectors. (E) Phase-contrast image of a representative H9 cell colony transduced with APOA-II-GFP lentivector. (F) Karyotype analysis of H9 cells transduced with APOA-II-GFP lentivector.
Yang et al. BMC Biology 2013 11:86 doi:10.1186/1741-7007-11-86