Open Access Research article

Abcb4 acts as multixenobiotic transporter and active barrier against chemical uptake in zebrafish (Danio rerio) embryos

Stephan Fischer12, Nils Klüver3, Kathleen Burkhardt-Medicke34, Mirko Pietsch3, Anne-Marie Schmidt3, Peggy Wellner3, Kristin Schirmer125 and Till Luckenbach3*

Author Affiliations

1 Department of Environmental Toxicology, Eawag, Swiss Federal Institute of Aquatic Science and Technology, 8600 Dübendorf, Switzerland

2 Department of Environmental Systems Sciences, ETH Zürich, Institute of Biogeochemistry and Pollutant Dynamics, 8092 Zürich, Switzerland

3 Department of Bioanalytical Ecotoxicology, UFZ – Helmholtz Centre for Environmental Research, 04318 Leipzig, Germany

4 Institute of Hydrobiology, Dresden University of Technology, 01062 Dresden, Germany

5 Laboratory of Environmental Toxicology, EPF Lausanne, School of Architecture, Civil and Environmental Engineering, 1015 Lausanne, Switzerland

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BMC Biology 2013, 11:69  doi:10.1186/1741-7007-11-69

Published: 17 June 2013

Additional files

Additional file 1:

Additional information regarding the qPCR analysis procedure: MIQE (Minimum Information for Publication of Quantitative Real-Time PCR Experiments) checklist. Table S1 with percent similarity data from sequence comparisons. The table is supplementary to Figure 1B. Table S2 with the accession nos. of sequences used for phylogenetic analyses. The table is supplementary to Figure 1B. Table S3 with RhB amounts that had accumulated in 1, 6, 12, 24 and 48 hpf zebrafish embryos upon co-exposure to various compounds. The table is supplementary to Figures 4 and 6. Table S4 with values from concentration-effect curves determined in zebrafish embryo toxicity experiments. The table is supplementary to Figure 7. Table S5 with sequences of primers used for qPCR. The table is supplementary to Table 1. Table S6 with efficiencies of zebrafish abcb4, abcb5 and housekeeping primers used in qPCR reactions. The table is supplementary to Table 1. Table S7 with primer pairs used for PCR of zebrafish abcb4 fragments used for generating probes for whole-mount in situ hybridization (WISH). The table is supplementary to Figure 2. Figure S1 with conserved synteny of abcb1/ABCB1 and abcb4/ABCB4 regions in various species. Figure S2 with Ct values determined for housekeeping gene candidates in different embryo stages of zebrafish with qPCR. The figure is supplementary to Table 1. Figure S3 with images of 120 hpf zebrafish embryos with abcb4 mRNA transcripts visualized using WISH. The figure is supplementary to Figure 2. Figure S4 with a standard curve used to determine the amount of RhB taken up by zebrafish embryos. The figure is supplementary to Figures 4 and 6. Figure S5 with images of Western blots with recombinant zebrafish Abcb4 protein obtained with the baculovirus expression system. Figure S6 with results of experiments proving the functionality of the used morpholinos.

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