Additional file 2.
13C Nuclear magnetic resonance spectroscopy (NMR) spectra of Plasmodium falciparum-infected and uninfected red blood cell (RBC) culture medium. Early trophozoite-infected RBCs (A), gametocytes (G), and uninfected RBCs (R) were cultivated in medium containing 8 mmol/l 13C-U-glucose. Uptake of 13C-glucose, and secretion of 13C-lactate and 13C-acetate was monitored by 13C-NMR analysis of the medium over 24 hours and compared with the initial medium (t0) and medium lacking RBCs at 24 hours (M). Representative spectra, with peaks corresponding to glucose, lactate, and acetate, are shown. ‘L’ represents the second lactate peak cluster, and a magnified area corresponding to acetate (inset) is shown. Abundances are shown in arbitrary units on an equivalent y-axis, with absolute quantification shown in Figure 2A.
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MacRae et al. BMC Biology 2013 11:67 doi:10.1186/1741-7007-11-67