Figure 3.

Comparison of MCPH1 enhancing and repressing effects between humans and macaques. (A) The enhancing effect difference between hMCPH1 and mMCPH1 on the CyclinE1 promoter. (B) The enhancing effect difference between hMCPH1 and mMCPH1 on the p73 promoter. (C) The enhancing effect difference between hMCPH1 and mMCPH1 on the p14ARF promoter. For enhancing effect assay, 0.2 ug target gene promoter construct was co-transfected with 0.2 ug HA tagged E2F1 per/well or 0.2 ug HA tagged E2F1 and 0.2 ug HA tagged hMCPH1 per/well or 0.2 ug HA tagged E2F1 and 0.2 ug HA tagged mMCPH1 per well in 24-well plates in HEK923T cells, Renilla was used as the internal control. The HA-tagged empty vector was used as the negative control. (D) The repressing effect difference between hMCPH1 and mMCPH1 on the CyclinE1 promoter. (E) The repressing effect difference between hMCPH1 and mMCPH1 on the p73 promoter. (F) The repressing effect difference between hMCPH1 and mMCPH1 on the p14ARF promoter. For repression assay, 0.2 ug target gene promoter construct was co-transfected with 0.2 ug HA tagged hMCPH1 per/well or 0.2 ug HA tagged mMCPH1 per/well in 24-well plates in HEK923T cells, Renilla was used as the internal control. The HA-tagged empty vector was used as the negative control. All histograms represent the mean ± SD of at least nine data points including both biological and technical replicates (* P <0.05,** P <0.01, *** P <0.001, n.s, not significant).

Shi et al. BMC Biology 2013 11:62   doi:10.1186/1741-7007-11-62
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