Behavior of B. thetaiotaomicron and F. prausnitzii in combination in vitro and in vivo. (A) Acetate, butyrate and propionate concentrations from culture media, pure cultures of B. thetaiotaomicron (Bt), F. prausnitzii (Fp) and cocultures. (B) Establishment of B. thetaiotaomicron and F. prausnitzii in the gastrointestinal tract (GIT) of Bt + Fp-30d rats (n = 16) was monitored weekly by enumeration in the feces; arrows represent the various inoculations with F. prausnitzii before successful colonization. (C) Cecal concentration of short chain fatty acids (SCFA) in the cecum of germfree (GF) (n = 12), Bt-30d rats (n = 19), and Bt + Fp-30d rats (n = 16). (D) Measurement of oxidoreduction potential (mV) in the cecal contents of GF (n = 11), Bt-2d (n = 13), Bt-30d (n = 19) and Bt + Fp-30d rats (n = 16). Means with different letters are significantly different (P-value <0.05).
Wrzosek et al. BMC Biology 2013 11:61 doi:10.1186/1741-7007-11-61