Open Access Research article

Bacteroides thetaiotaomicron and Faecalibacterium prausnitzii influence the production of mucus glycans and the development of goblet cells in the colonic epithelium of a gnotobiotic model rodent

Laura Wrzosek123, Sylvie Miquel123, Marie-Louise Noordine123, Stephan Bouet456, Marie Joncquel Chevalier-Curt789, Véronique Robert123, Catherine Philippe1102, Chantal Bridonneau123, Claire Cherbuy123, Catherine Robbe-Masselot789, Philippe Langella123* and Muriel Thomas123*

Author Affiliations

1 INRA, UMR 1319 MICALIS, AgroParisTech, Domaine de Vilvert, Jouy-en-Josas, 78350, France

2 AgroParisTech, UMR 1319 MICALIS, Jouy-en-Josas, F-78350, France

3 Commensal and Probiotics-Host Interactions Laboratory, Jouy-en-Josas, F-78350, France

4 INRA, UMR 1313, Animal Genetics and Integrative Biology, Jouy-en-Josas, F-78350, France

5 Laboratory of Radiobiology and Genomics Studies, CEA, DSV, IRCM, SREIT, Jouy-en-Josas, F-78350, France

6 AgroParisTech, UMR 1313, Animal Genetics and Integrative Biology, Jouy-en-Josas, F-78350, France

7 Univ Lille Nord de France, Lille, F-59000, France

8 USTL, UGSF, IFR 147, CNRS, Villeneuve d’Ascq, F-59650, France

9 UMR 8576, Villeneuve d’Ascq, F-59650, France

10 Amipem Team, Jouy-en-Josas, F-78350, France

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BMC Biology 2013, 11:61  doi:10.1186/1741-7007-11-61

Published: 21 May 2013

Additional files

Additional file 1: Figure S1:

Characterization of the colonic epithelial response in B. thetaiotaomicron and F. prausnitzii di-associated rats. (A) Representative pictures of colonic sections stained with HES in GF, Bt-2d, Bt-30d and Bt + Fp-30d rats. Representative pictures showing goblet cells staining with (B) alcian blue (indicated as AB) and (C) periodic acid Schiff (indicated as PAS) in Bt-30d rats and Bt + Fp 30d (n = 3) rats. Scale bars, 50 μm.

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Additional file 2: Table S1:

Deduced monosaccharide composition of mucin O-glycans from GF, Bt-2d, Bt-30d and Bt + Fp 30d rats, as identified by MALDI-TOF mass spectrometry in positive ion mode. The relative proportions of each oligosaccharide were calculated by integration of peaks on MS spectra. Mucus O-glycans types were separated into neutral oligosaccharides (blue), oligosaccharides carrying sialic acid (orange) and sulfated residues (green). Results are presented as means ± SD of the percentage of each oligosaccharide.

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