N-terminal sequence of Peak III peptides (Relocator) confirms identity with crotatroxins (CT) 1 and 2. Note that CTs 1 and 2 are identical in sequence except for the additional N-terminal alanine residue in CT1. Protein sequencing of the relocator peak showed lower yield (approximately 3 pmol, compared to approximately 6.5 pmol for residues 2 to 6) and presence of an N-terminal alanine at residue 1, indicating that both CTs were present. No secondary sequence (indicative of potential contaminant proteins) was observed.
Saviola et al. BMC Biology 2013 11:20 doi:10.1186/1741-7007-11-20