Peak III consist only of 7 kDa peptides. (A) Reversed-phase chromatography of Peak III from the gel filtration step (BioGel P-100). Two hundred microliters was injected onto a Vydac C18 (4.6 × 250 mm) column, and disintegrin peaks were eluted at 23% buffer B (13 to 14 minutes). (B) MALDI-TOF-MS analysis of crotatroxin 1 from the reverse-phase chromatography purification step (fraction 13). Mass of 7,440.35 was observed for crotatroxin 1. (C) MALDI-TOF-MS analysis of crotatroxin 2 from the reverse-phase chromatography purification step (fraction 14). Mass of 7,383.29 was observed for crotatroxin 2.
Saviola et al. BMC Biology 2013 11:20 doi:10.1186/1741-7007-11-20