Decreased apoptotic cell death in Amhr2-Cre/+;Notch2flox/- ovaries. (A, B) Quantification of terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (TUNEL)-positive (A) oocytes and (B) pre-granulosa/granulosa cells (defined as a TUNEL-positive somatic cell adjacent to a Vasa-positive oocyte). Data are presented as the mean ± SD of four ovaries from four individual mutant or control mice. At postnatal day (PND)1, there were significant differences in the numbers of both apoptotic (that is, TUNEL-positive) oocytes and pre-granulosa/granulosa cells between the control and the Amhr2-Cre/+;Notch2flox/- ovaries. * P < 0.05; ** P < 0.01. (C, D) Representative micrographs showing TUNEL staining from (C) control littermate and/(D) Amhr2-Cre/+;Notch2flox ovaries at PND1. TUNEL-positive cells are in green; oocytes are in red (Vasa-positive), with nuclear DAPI staining in blue. (E) Higher magnification view of the mutant ovary (boxed region in D). Arrow: TUNEL-positive oocyte; arrowhead: two TUNEL-positive pre-granulosa/granulosa cells adjacent to a Vasa-positive oocyte.
Xu and Gridley BMC Biology 2013 11:13 doi:10.1186/1741-7007-11-13