Absence of extended cell proliferation in neonatal Amhr2-Cre/+;Notch2flox/- ovaries. (A, B) Quantification of (A) total 5-bromo-2'-deoxyuridine (BrdU)-positive cells and (B) BrdU-positive pre-granulosa/granulosa cells (defined as a BrdU-positive somatic cell adjacent to a Vasa-positive oocyte). Ovaries were harvested from Amhr2-Cre/+;Notch2flox/- and control littermate mice after a 2-hour BrdU pulse administration on postnatal day (PND)1 or PND2. Data are presented as the mean ± SD of four ovaries from four individual mutant or control mice. There were no significant differences in the numbers of either (A) total proliferating cells or (B) proliferating pre-granulosa/granulosa cells between the control and the Amhr2-Cre/+;Notch2flox/- ovaries. (C, D) Representative micrographs showing BrdU incorporation from (C) control littermate and (D) Amhr2-Cre/+;Notch2flox/- ovaries at PND1. No oocyte in either (C) the control or (D) mutant ovary incorporated BrdU. BrdU-positive cells are in green; oocytes are in red (Vasa-positive), with nuclear DAPI staining in blue. (E) Higher magnification view of the mutant ovary (boxed region in D).
Xu and Gridley BMC Biology 2013 11:13 doi:10.1186/1741-7007-11-13