Determination of mycothiol production in Corynebacterium glutamicum wild-type and different mutants and complemented strains. The mycothiol was derivatized using bromobimane, separated by high-performance liquid chromatography (HPLC) and monitored using a fluorescence detector (390 nm excitation and 475 nm emission). As control, the mycothiol deficient strain ΔmshC was used as well as a wild-type sample that had been treated with N-methyl-maleimide (NMM) to block thiols prior to derivatization. The peak assumed to be mycothiol is marked with a dotted line. Presented is a representative chromatogram of three biological replicates each.
Baumgart et al. BMC Biology 2013 11:122 doi:10.1186/1741-7007-11-122