Tracheal epithelial differentiation and apoptosis was not altered by lack of Sox9 gene. (A, B) Phospho-histone-3 (pH3) staining on longitudinal sections of (A) wild-type and (B) mutant tracheas at embryonic day (E) 15.5 did not reveal any change in epithelial cell proliferation. (C) Number of pH3-positive cells per section was similar between wild-type and mutant mouse trachea. (D) Real-time PCR for the apoptotic markers p53, Bcl2, and Gadd45a on total RNA extracted from E15.5 wild-type and mutant tracheas indicated no change in apoptosis. (E) Tracheal epithelial cells were counted on transverse sections of Sox9fl/fl and Sox9Δ/Δ E18.5 tracheas, revealing no change in cell number between the two groups. Values are mean ± SD.
Turcatel et al. BMC Biology 2013 11:117 doi:10.1186/1741-7007-11-117