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standard / ## Figure 5.
The number of DNA double strand breaks determines the probability of a p53 pulse.
(A) Schematic representation of the experimental approach. Cells treated with low doses
of NCS (0 to 100 ng/ml) were imaged for 8hr. Induced DSBs and p53 dynamics were quantified.
(B) On the resulting data, we performed a robust linear regression (blue line) and calculated
the corresponding confidence interval (α = 0.05, shaded blue area). For visualization,
cells were binned according to the number of induced DSBs (bin size W = 4 foci) and
the fraction of cells inducing a p53 pulse was plotted for each bin (blue dots). The
number of cells in each bin is indicated by grey bars (total number of cells = approximately
350). DSBs, couble strand breaks; NCS, neocarcinostatin.
Loewer |