Human cell lines show a series of p53 pulses in response to DSBs. (A-C) The p53-Venus reporter was expressed in three lines: MCF7 - breast cancer (A); A549 - lung cancer (B); and RPE1 - retinal epithelial, non-cancerous (C). Shown are representative examples of p53 trajectories in individual cells following DSBs (10Gy γ-irradiation). (D) MCF7 cells expressing the p53-Venus reporter were treated with the indicated doses of neocarcinostatin (NCS) and the number of p53 pulses post-damage was quantified. In each condition, the p53 response shows a high degree of heterogeneity. Number of cells >100 for each condition. DSBs, double strand breaks.
Loewer et al. BMC Biology 2013 11:114 doi:10.1186/1741-7007-11-114