Validation of the DSB reporter cell line. (A) Distributions of the number of 53BP1-mCherry foci at 18 hours post damage in cells imaged every hour for 18 hours (i) or imaged only once at 18 hours (ii) post damage. Number of cells = approximately 140 for each condition; P-value 0.41, Kolmogorov-Smirnov test. (B) Time-lapse images of two cells that were pre-treated with a small molecule inhibitor of DNA-PK (NU7026) prior to 5Gy γ-irradiation. (C) Number of DSBs over time in cells treated with 5Gy γ-irradiation alone (blue) or in the presence of DNA-PK inhibitor (red). Solid lines represent the median, shaded areas the 25th to 75th percentile. Number of cells = 97 (5Gy) and n = 81 (5Gy + inhibitor). DSB, double strand break.
Loewer et al. BMC Biology 2013 11:114 doi:10.1186/1741-7007-11-114