Both myofibroblasts (SMA positive) and lipofibroblasts (ADRP positive) were marked by the Tbx4 lung enhancer driven Tet-On system. E18.5 lung tissue sections of triple transgenic mice (Tbx4-rtTA/TetO-Cre/mT-mG) with different Dox induction during early or late gestation as indicated were co-stained with mGFP/ADRP (A) or mGFP/SMA (B). Cell nuclei, counter-stained with DAPI (blue), were not included in the merged panels. ADRP, adipophilin; DAPI, 4',6-diamidino-2-phenylindole; Dox, doxycycline; E, embryonic day; SMA, α-smooth muscle actin.
Zhang et al. BMC Biology 2013 11:111 doi:10.1186/1741-7007-11-111