Open Access Open Badges Research article

Requirement for integrin-linked kinase in neural crest migration and differentiation and outflow tract morphogenesis

Xiuqin Dai1, Weijian Jiang2, Qingquan Zhang1, Lian Xu1, Peng Geng1, Shaowei Zhuang1, Brian G Petrich3, Cizhong Jiang4, Luying Peng1, Shoumo Bhattacharya5, Sylvia M Evans3, Yunfu Sun1*, Ju Chen3* and Xingqun Liang13*

Author Affiliations

1 Key Laboratory of Arrhythmia, Ministry of Education, East Hospital, Tongji University School of Medicine, 150 Jimo Road, Shanghai 200120, China

2 New Era Stroke Care and Research Center, The Second Artillery General Hospital PLA, Beijing 100088, China

3 Department of Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA

4 School of Life Sciences and Technology, Tongji University, Shanghai 200120, China

5 Department of Cardiovascular Medicine, University of Oxford, Oxford, UK

For all author emails, please log on.

BMC Biology 2013, 11:107  doi:10.1186/1741-7007-11-107

Published: 16 October 2013

Additional files

Additional file 1: Figure S1:

Expression of integrin-linked kinase (ILK) in the outflow tract (OFT) and neural crest cells (NCCs). (A, B’) Wholemount in situ hybridization show ubiquitous ILK expression in E8.5 and E9.5 embryos. (C-E) Immunostaining with ILK antibody and β-gal staining of adjacent sections from Wnt1-Cre; R26R embryos at E9.5 show that ILK expression in cardiac NCC in the OFT. (F-H) Immunostaining with antibodies to ILK and Sox10 show ILK expression in cultured NCCs. (I-L) ILK is not detected in cardiac NCC-derived OFT mesenchyme in E9.5 NKO mutants.

Format: TIF Size: 5.7MB Download file

Open Data

Additional file 2: Table S1:

Microarray analysis of neural crest cells from the outflow tract (OFT) of control and integrin-linked kinase (ILK) mutant embryos at E10.5. Microarray and quantitative polymerase chain reaction (qPCR) analysis of neural crest cells from the OFT of control and ILK mutant embryos at E10.5.

Format: PDF Size: 55KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data

Additional file 3: Table S2:

Quantitative polymerase chain reaction (qPCR) primer list. List of qPCR primers used in this study.

Format: PDF Size: 43KB Download file

This file can be viewed with: Adobe Acrobat Reader

Open Data