Open Access Research article

Requirement for integrin-linked kinase in neural crest migration and differentiation and outflow tract morphogenesis

Xiuqin Dai1, Weijian Jiang2, Qingquan Zhang1, Lian Xu1, Peng Geng1, Shaowei Zhuang1, Brian G Petrich3, Cizhong Jiang4, Luying Peng1, Shoumo Bhattacharya5, Sylvia M Evans3, Yunfu Sun1*, Ju Chen3* and Xingqun Liang13*

Author Affiliations

1 Key Laboratory of Arrhythmia, Ministry of Education, East Hospital, Tongji University School of Medicine, 150 Jimo Road, Shanghai 200120, China

2 New Era Stroke Care and Research Center, The Second Artillery General Hospital PLA, Beijing 100088, China

3 Department of Medicine, University of California San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA

4 School of Life Sciences and Technology, Tongji University, Shanghai 200120, China

5 Department of Cardiovascular Medicine, University of Oxford, Oxford, UK

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BMC Biology 2013, 11:107  doi:10.1186/1741-7007-11-107

Published: 16 October 2013

Additional files

Additional file 1: Figure S1:

Expression of integrin-linked kinase (ILK) in the outflow tract (OFT) and neural crest cells (NCCs). (A, B’) Wholemount in situ hybridization show ubiquitous ILK expression in E8.5 and E9.5 embryos. (C-E) Immunostaining with ILK antibody and β-gal staining of adjacent sections from Wnt1-Cre; R26R embryos at E9.5 show that ILK expression in cardiac NCC in the OFT. (F-H) Immunostaining with antibodies to ILK and Sox10 show ILK expression in cultured NCCs. (I-L) ILK is not detected in cardiac NCC-derived OFT mesenchyme in E9.5 NKO mutants.

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Additional file 2: Table S1:

Microarray analysis of neural crest cells from the outflow tract (OFT) of control and integrin-linked kinase (ILK) mutant embryos at E10.5. Microarray and quantitative polymerase chain reaction (qPCR) analysis of neural crest cells from the OFT of control and ILK mutant embryos at E10.5.

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Additional file 3: Table S2:

Quantitative polymerase chain reaction (qPCR) primer list. List of qPCR primers used in this study.

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