Table 4

Primers used for reporter-gene retrofitting
Gene Primers (5′-3′) Sequencea
AMOTL1 Forward CCGGCAGCCGTCTTCCCCAGCCGAGGGACTGAACTAGCCATGATCGCCTCatggcggatcccgtcgtttt
Reverse GTGGGAGACTCGGAGACGCCCTCCCGGCACCTCGAGTGGGGGCTGGTTACgaagttcctatactttctag
LCT Forward TTGCAGTTATAAAGTAAGGGTTCCACATACCTCCTAACAGTTCCTAGAAAatggtgagcaagggcgagga
Reverse TGTGTGATGAAGGTTGCCGAGGGGTCACCATCAGGTCAATGTGTACTCACgaagttcctatactttctag
MAOA Forward TTGCCGTCCCCACTCCTGTGCCTACGACCCAGGAGCGTGTCAGCCAAAGCatggcggatcccgtcgtttt
Reverse ACCCCTCACTGGCCAGGGTCCCCCAGGCCACCGCTACGGTCCACACTGACgaagttcctatactttctag
NGFR Forward CCGCAAAGCGGACCGAGCTGGAAGTCGAGCGCTGCCGCGGGAGGCGGGCGatggcggatcccgtcgtttt
Reverse GGAGTTCTGATCCCGGGAAAGGGAGCGGGCCCCCTCCGGCTAACACTCACgaagttcctatactttctag
NOV Forward TACAGCGAAGAAAGTCTCGTTTGGTAAAAGCGAGAGGGGAAAGCCTGAGCatggcggatcccgtcgtttt
Reverse ACCAAGGCGGGCAAAGTAACTTGGGGGCATCTTAAGGGTGTGCCACTTACgaagttcctatactttctag
NR2E1 Forward GCCGGGACTCGGGCAGCGCCCACCAACCGCTCCGCCCCGGGACAGCCAGCatggcggatcccgtcgtttt
Reverse TCGCCCCAGGCTGCGCGCCTAGGCCCCACGGCGGCCCGAGAGGTACCCACgaagttcctatactttctag
NR2F2 Forward CGCCGCCCGCAGCCAGGGGAGCAGGAAGTCCGGACGCAGCCCCCATAGATatggcggatcccgtcgtttt
Reverse CCAGGACCCCGGGACCCAGGACGAGGGAAGGAGAAATGAGAGGCCGATACgaagttcctatactttctag
PITX2 Forward CCGCCGCTTCTTACAGCCTTCCTTCTCTTCTGTTTTGCAGATAACGGGGAatggcggatcccgtcgtttt
Reverse GTGGCGCGGCCTCCCGTCCGATGACCCGGGCAGGAGAAGGGGGTTCTTACgaagttcctatactttctag

aCapital letters, sequence matching the mouse genome; lowercase letters, sequence matching the reporter gene (forward primer) or the kanamycin cassette (reverse primer).

Schmouth et al.

Schmouth et al. BMC Biology 2013 11:106   doi:10.1186/1741-7007-11-106

Open Data