Table 1

Eight out of ten constructs successfully modified for expression-pattern characterization in mice
Brain region(s) Gene Plea Parental BAC name Construct size (bp) Reporter BAC name Retrofitting status
Thalamus AMOTL1 5 RP11-936P10 211,681 lacZ bEMS90 Successful
Brainstem, pons, medulla GLRA1 91 RP11-602K10 188,541 N/A bEMS89 Failedb
Hippocampus, dentate gyrus LCT 126 RP11-406M16 156,570 EGFP bEMS75 Successful
Locus coeruleus MAOA 127 RP11-475M12 197,191 lacZ bEMS84 Successful
Hippocampus, Ammon’s horn NEUROD6 132 RP11-463M14 N/A N/A N/A Failedc
Basal nucleus of Meynert NGFR 133 RP11-158L10 175,851 lacZ bEMS88 Successful
Amygdala, basolateral complex NOV 134 RP11-840I14 202,342 lacZ bEMS91 Successful
Neurogenic regions NR2E1 142 RP11-144P8 138,165 lacZ bEMS86 Successful
Amygdala, basolateral complex NR2F2 143 RP11-134D15 213,112 lacZ bEMS85 Successful
Subthalamic nucleus PITX2 158 RP11-268I1 200,209 lacZ bEMS87 Successful

aPleiades (Ple) promoter design number.

bGLRA1 failed retrofitting with Hprt homology arms.

cNEUROD6 failed BAC isolation from the library.

BAC bacterial artificial chromosome, N/A not applicable.

Schmouth et al.

Schmouth et al. BMC Biology 2013 11:106   doi:10.1186/1741-7007-11-106

Open Data