Human AMOTL1-lacZ expressed in mature neurons in the thalamus in adult brain, amacrine and ganglion cells in adult retina, and major components of the vasculature system during development. Expression analysis of the human AMOTL1-lacZ strain was examined using β-galactosidase (β-gal) histochemistry staining (blue). (a) E12.5 whole embryos stained in the jugular vein (black arrow), posterior cerebral artery (white arrow), and vertebral arteries (black arrowhead). (b) E12.5 cleared embryos additionally demonstrated staining in the basilary artery (black arrow), the dorsal aorta (white arrow), the omphalomesenteric vascular system (white arrowhead), and the lateral nasal prominence (black arrowhead). (c) P7 brains stained in the developing anterior thalamic nuclei (black arrows), and hippocampal formation (HPF) (white arrows). (d) Adult brains stained in the anteromedial and anteroventral thalamic nuclei (black arrow), and the pontine nuclei (white arrow). (e) The HPF showed sharp staining in the cornu ammonis fields (CA1, and CA3). Staining was also present in the dorsal subiculum and extended to the lower cortical layers with pronounce staining in layer VI. (f) Colocalization experiment using β-gal staining and neuronal nuclei (NeuN) immunohistochemistry (brown) performed on adult brain cryosections demonstrated expression of AMOTL1-lacZ in thalamic neurons. (g) Staining was detected in two layers of the adult retina, the inner limiting membrane (ILM) (black arrows), and the junction between the inner plexiform layer (IPL) and the ILM. (h) Colocalization experiment using β-gal staining and an anti-tyrosine hydroxylase (anti-TH) antibody (brown) performed on adult eye cryosections indicated colocalization of AMOTL1-lacZ in amacrine cells populating the inner nuclear layer (INL) (black arrows). (i) Colocalization experiment using β-gal staining and an anti-Brn3 antibody (brown) performed on adult eye cryosections indicated colocalization of AMOTL1-lacZ in ganglion cells populating the ganglion cell layer (GCL) (black arrows). AV, anteroventral thalamic nucleus; ctx, cortex; Hip, hippocampus; ONL, outer nuclear layer; SUB, subiculum. Scale bar: (a-d) 1 mm; (e) 500 μm; (f) 100 μm; (g-i) 20 μm.
Schmouth et al. BMC Biology 2013 11:106 doi:10.1186/1741-7007-11-106