Additional file 1.

Diagram of transgene constructs. Schematics showing pertinent details of the transgenes tested and corresponding acronyms. Core elements include: cfos - minimal promoter [34]; KalTA4 - an optimized Gal4-VP16 fusion protein [43]; GI - rabbit beta-globin intron to promote mRNA stability [89]; pA - SV40 or bovine growth hormone polyadenylation sequences; 2xNRSE - a tandem repeat of a 21 bp consensus NRSE site [33], lox - loxP recombination sites to allow transgene cassette swapping [92]; UAS - 17 bp upstream activator sequence [63] specific for the Gal4 DNA binding domain (with indicated number of repeats, e.g., 5x or 14x); E1b - a basal promoter from carp beta-actin [37]; CREST1 - a 800-bp enhancer element characterized as a cranial motor neuron-specific element [35]; 2A - a porcine 2A viral peptide sequence [86] promoting equimolar expression of multicistronic messages [87]; nfsB - Escherichia coli gene encoding the prodrug converting bacterial enzyme nitroreductase (Ntr) which promotes chemically-induced cell ablation [52,53,88]; HE - a 365-bp promoter element from the zebrafish hatching enzyme 1a locus (he1a) that allows facile detection of UAS reporter lines in the absence of Gal4-VP16 driver elements (see Additional file 5). Fluorescent reporters included: M-YFP - a membrane-tagged (dual palmitoylation sequence from the Xenopus gap43 locus [93] 'enhanced' yellow fluorescent protein (EYFP); M-tYFP - a membrane-tagged (same as above) monomeric 'tag' yellow fluorescent protein (tagYFP); mCherry - a monomeric red fluorescent protein [94].

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Xie et al. BMC Biology 2012 10:93   doi:10.1186/1741-7007-10-93