Table 3

Confidence score of predicted gene ontology terms

Evaluation method

Criteria

Points


BLAST e-value

0.001

1


Sequence identity between the query and the hit

identity 30% to 50%, coverage > 40%

1


identity 50% to 70%, coverage > 40%

2


identity 70% to 90%, coverage > 40%

3


identity 90% to 100%, coverage > 40%

4


Alignment coverage for query and hit

60% to 80%

1


80% to 100%

2


Evidence code of the GO term assigned to the hit

EXP, IDA

3


IPI, IMP, IGI, IEP, ISO, TAS

2


ISS, ISA, ISM, IGC, IBA, IBD, IKR, IRD, RCA, NAS, IC, IEA

1


Consensus bonus

Associated with no less than three hits

2


EXP: inferred from experiment; GO: Gene Ontology; IBA: inferred from biological aspect of ancestor; IBD: inferred from biological aspect of descendant; IC: inferred by curator; IDA: inferred from direct assay; IEA: inferred from electronic annotation; IEP: inferred from expression pattern; IGC: inferred from genomic context; IGI: inferred from genetic interaction; IKR: inferred from key residues; IMP: inferred from mutant phenotype; IPI: inferred from physical interaction; IRD: inferred from rapid divergence; ISA: inferred from sequence alignment; ISM: inferred from sequence model; ISO: inferred from sequence orthology; ISS: inferred from sequence or structural similarity; NAS: non-traceable author statement; RCA: inferred from reviewed computational analysis; TAS: traceable author statement.

Cong and Grishin BMC Biology 2012 10:82   doi:10.1186/1741-7007-10-82

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