Email updates

Keep up to date with the latest news and content from BMC Biology and BioMed Central.

Journal App

google play app store
Open Access Highly Accessed Open Badges Methodology article

Rapid production of antigen-specific monoclonal antibodies from a variety of animals

Nobuyuki Kurosawa1, Megumi Yoshioka2, Rika Fujimoto3, Fuminori Yamagishi4 and Masaharu Isobe1*

Author Affiliations

1 Laboratory of Molecular and Cellular Biology, Faculty of Science and Engineering, Graduate School, University of Toyama, 3190 Gofuku, Toyama-shi, Toyama, 930-8555, Japan

2 Graduate School of Innovative Life Science, University of Toyama, Toyama-shi, Toyama, 930-8555, Japan

3 Department of Immunology, Kochi Medical School, Nangoku-shi, Kochi, 783-8505, Japan

4 Department of Surgery, Itoigawa General Hospital, Itoigawa-shi, Niigata, 941-0006, Japan

For all author emails, please log on.

BMC Biology 2012, 10:80  doi:10.1186/1741-7007-10-80

Published: 28 September 2012



Although a variety of animals have been used to produce polyclonal antibodies against antigens, the production of antigen-specific monoclonal antibodies from animals remains challenging.


We propose a simple and rapid strategy to produce monoclonal antibodies from a variety of animals. By staining lymph node cells with an antibody against immunoglobulin and a fluorescent dye specific for the endoplasmic reticulum, plasma/plasmablast cells were identified without using a series of antibodies against lineage markers. By using a fluorescently labeled antigen as a tag for a complementary cell surface immunoglobulin, antigen-specific plasma/plasmablast cells were sorted from the rest of the cell population by fluorescence-activated cell sorting. Amplification of cognate pairs of immunoglobulin heavy and light chain genes followed by DNA transfection into 293FT cells resulted in the highly efficient production of antigen-specific monoclonal antibodies from a variety of immunized animals.


Our technology eliminates the need for both cell propagation and screening processes, offering a significant advantage over hybridoma and display strategies.

antigen-specific monoclonal antibody; ER-tracker; ERIAA; FACS; guinea pig; human; MAGrahd; rabbit; rat; single cell; TS-jPCR