Open Access Research article

The embryonic leaf identity gene FUSCA3 regulates vegetative phase transitions by negatively modulating ethylene-regulated gene expression in Arabidopsis

Shelley Lumba1, Yuichiro Tsuchiya1, Frederic Delmas1, Jodi Hezky1, Nicholas J Provart13, Qing Shi Lu12, Peter McCourt1 and Sonia Gazzarrini12*

Author Affiliations

1 Department of Cell and Systems Biology (CSB), University of Toronto, 25 Willcocks Street, Toronto, ON, M5S 3B2, Canada

2 Department of Biological Sciences, University of Toronto at Scarborough, 1265 Military Trail, Toronto, ON, M1C 1A4, Canada

3 Centre for the Analysis of Genome Evolution and Function (CAGEF), University of Toronto, 25 Harbord Street, Toronto, ON, M5S 3G5, Canada

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BMC Biology 2012, 10:8  doi:10.1186/1741-7007-10-8

Published: 20 February 2012

Additional files

Additional file 1:

Table S1 Genes upregulated by ectopic FUS3 activation. Values for fold changes in expression after 2 and 4 days (d) of FUS3 activation with dexamethasone (+DEX) are the averages of two replicates. The presence of RY promoter motifs (CATGCA) in the 500 bp (0.5 K), 1, 000 bp (1 K) and 3, 000 bp (3 K) upstream regions of each gene is included. p-values are 3.06 × 10-4, 1.77 × 10-4 and 2.57 × 10-2, respectively.

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Additional file 2:

Table S2 Genes downregulated by ectopic FUS3 activation. Values for fold changes in expression after 2 and 4 days (d) of FUS3 activation with dexamethasone (+DEX) are the averages of two replicates. Genes involved in hormone metabolism or response are in bold. Ethylene response genes that were downregulated less than twofold by FUS3 activation at both 2 and 4 days are in italics. The presence of RY promoter motifs (CATGCA) in 500 bp (0.5 K), 1, 000 bp (1 K) and 3, 000 bp (3 K) upstream regions of each gene is included. p-values are 4.27 × 10-2, 6.06 × 10-3 and 3.46 × 10-2, respectively.

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Additional file 3:

Figure S1 Phenotypic analysis of wild-type plants exposed to dexamethasone (DEX). Wild-type seeds were germinated in 10 μM DEX for 2 days and then transferred to soil. The increase in the blade-to-petiole ratio and the appearance of the abaxial trichomes on leaf 5 is comparable to wild-type profiles shown in Figure 3. (A) Ratios of blade-to-petiole lengths of individual wild-type rosette leaves. (B) Percentage of wild-type rosettes that formed abaxial trichomes at each leaf position.

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