Additional file 5.
Effect of media supplementation with folate on C. elegans lifespan on aroD and ubiG mutants. (A) glp-4(bn2) animals were raised on the aroD mutant until L4 (15°C) and then transferred (25°C) to the aroD mutant (n = 100), control (n = 126), aroD + 1 × folate (n = 101), aroD + 2 × folate (n = 149). aroD vs aroD + 1 × folate, P = < 0.0001; aroD + 1 × folate vs aroD + 2 × folate, P = 0.13 (Log Rank), P = 0.055 (Wilcoxon). (B) glp-4 worms were raised on E. coli OP50 (15°C) and transferred (25°C) at L4 to ubiG (n = 145), ubiG + pAHG (ubiG+ rescue plasmid), GD1 + 1 × folate (n = 145), GD1+ 2 × folate (n = 146). (C) Traces from the HPLC/MS analysis of the wild type (HT115(DE3)) and aroD mutant (HT115(DE3)aroD) bacteria. Peaks shown are m/z = 730.25 and 548.67 corresponding to formylTHFGlu3 and the methotrexateGlu6 spiked standard respectively with increasing folic acid supplementation (1 × folate is equal to 294 μM folic acid).
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Virk et al. BMC Biology 2012 10:67 doi:10.1186/1741-7007-10-67