Additional file 2.
Spontaneous E. coli mutant rather than RNAi plasmid extends C. elegans lifespan. A) A new RNAi strain containing the ugt-27 plasmid has no effect on lifespan. Survival of rrf-3 worms (20°C) on the control HT115(DE3) strain with the L4440 plasmid, (control, n = 61), the original ugt-27 strain (mutant, n = 98) and a new strain consisting of HT115(DE3) transformed with the ugt-27 plasmid (new, n = 50). Difference between mutant and control, 29.3% (P = < 0.0001).
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Virk et al. BMC Biology 2012 10:67 doi:10.1186/1741-7007-10-67