SMX has little effect on OP50 growth, viability or metabolome. A) in OD600 measurements of bacterial density show that SMX at 16 and 128 μg/ml has no effect on the log-phase growth rate of OP50 in liquid NGM at 37°C. B) Table showing colony forming units from bacteria scraped from lawns on agar plates, concentration of SMX used = 128 μg/ml. C) PLS-DA score plot showing two components that explain 58.9% (x-axis) and a further 11.9% (y-axis) of the variance between the conditions: whole LC-MS data from strains HT115(DE3) (labelled CWT), HT115(DE3)aroD (CM), OP50 (OP50) and OP50 treated with 128 μg/ml of SMX (SMX). Three replicates were performed for each condition.
Virk et al. BMC Biology 2012 10:67 doi:10.1186/1741-7007-10-67