Figure 3.

Inhibition of bacterial folate synthesis causes an increase in C. elegans lifespan. A) Treatment of OP50 with various concentrations of SMX increases mean C. elegans lifespan by the indicated percentage. Control (n = 102, 191), 0.1 μg/ml SMX (n = 139, 221), 1 μg/ml (n = 154, 229), 2 μg/ml (n = 152, 210), 4 μg/ml (n = 161, 226), 8 μg/ml (n = 146, 224), 16 μg/ml (n = 176, 226), 64 μg/ml (n = 235, 238), 128 μg/ml (n = 229, 230). 256 μg/ml (n = 253). B) SMX treatment decreases levels of formylTHFGlu3 in E. coli OP50 until it becomes undetectable at 2 μg/ml. * = below the level of detection. Two biological replicates are shown. C) The lifespan increase induced by 16 and 128 μg/ml SMX is eliminated when worms are maintained on OP50 containing the R26 plasmid that confers sulfonamide resistance. Control (n = 224), 16 μg/ml SMX (n = 218), 128 μg/ml SMX (n = 215).

Virk et al. BMC Biology 2012 10:67   doi:10.1186/1741-7007-10-67
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