Additional file 4.

Figure S4. LysoTracker Red (LTR) stain (red in left panels, grayscale in right panels) of single cell clones overexpressing dominant negative Tor (TorTED, green) in the buffyH37 mutant and wild-type background. Clones of TorTED in wild-type fat body (A) or buffyH37 fat body (B) from fed larvae are autophagic and small as expected. Similarly, clones of TorTED in wild-type fat body (C) or buffyH37 fat body (D) from larvae starved for 2 h are indistinguishable in their autophagic response. Note that in both cases, TorTED cells have the same amount of autophagy as neighboring Tor+ cells in the starved conditions. The TorTED clones observed in (C) and (D) were generated later in development as opposed to those observed in (A) and (B), which accounts for the difference is cell size. Images are representative of 7 to 10 different animals surveyed per genotype. Genotypes: (D, F) hs flp/+; UAS-TorTED/+; Act > CD2 > Gal4, UAS-GFP/+. (E, G) hs flp/+; buffyH37 , UAS-TorTED/buffyH37; Act > CD2 > Gal4, UAS-GFP/+.

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Monserrate et al. BMC Biology 2012 10:63   doi:10.1186/1741-7007-10-63