Additional file 3.

Figure S3. (A) Quantification of LysoTracker Red (LTR) stain from three different wild-type alleles to determine variation in autophagic responses to 2 h of starvation. Graph represents numbers from 11 different animals. The buffy mutant is included for comparison. (B) LC3-green fluorescent protein (GFP) marker highlights autophagic vacuoles to corroborate LTR data. Shown are fat bodies from 2 h-starved larvae expressing LC3-GFP (green, middle panels) and stained with LTR (red, top panels) with merged images shown in the bottom panels. More LC3-GFP punctae are observed in the buffyH37 mutant and wild-type larvae starved for 2 h. Yellow arrowheads point to examples of LC3-GFP punctae that colocalize with LTR (insets in wild-type images), indicating autolysosomes. Genotypes for (B): cg-Gal4, UAS-LC3-GFP and cg-Gal4, UAS-LC3-GFP, buffyH37.

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Monserrate et al. BMC Biology 2012 10:63   doi:10.1186/1741-7007-10-63