In situ detection of kinase activity with genetically encoded FRET reporter AKAR. A schematic diagram of the AKAR kinase activity reporter fused to the PKA and PDE4 binding sites of the A Kinase anchoring protein, mAKAP. The fluorescent moieties (CFP and YFP) are indicated. This FRET sensor detects changes in intracellular cAMP.
Scott and Newton BMC Biology 2012 10:61 doi:10.1186/1741-7007-10-61