Figure 2.

In situ detection of kinase activity with genetically encoded FRET reporter AKAR. A schematic diagram of the AKAR kinase activity reporter fused to the PKA and PDE4 binding sites of the A Kinase anchoring protein, mAKAP. The fluorescent moieties (CFP and YFP) are indicated. This FRET sensor detects changes in intracellular cAMP.