Table 2

Stage composition of Caenorhabditis populations

Sample ID

Substrate type

Substrate ID

Sample weight (total)

Population in plated sample

Total census (Log)

Population stage?

Species


O661

fruit

Rosaceae - Malus domestica

52 (94)

~ 30, all stages

2

P1

Cbr (9/9)

O662

fruit

Rosaceae - Malus domestica

25 (39)

~ 500, 20 ad, 300 d, 100 L2d, 100 L1

3

P3

Cbr (20/20)

O663

fruit

Rosaceae - Malus domestica

15 (23)

~ 20 (d?)

2

?

Cbr (12/12)

O664

fruit

Rosaceae - Malus domestica

10 (20)

~ 10,000, 250 ad, 7000 d, 3000 L2d

5

P3

Cbr (17/17)

O665

fruit

Rosaceae - Malus domestica

19 (32)

~ 2,000, 60 ad, 600 d, 600 L2d, 600 L1

4

P3

Cbr (18/18)

O666

fruit

Rosaceae - Malus domestica

27 (44)

~ 30 (d?)

2

?

Cbr (5/5)

O667

fruit

Rosaceae - Malus domestica

46 (83)

2 (1 ad, 1 L2d)

1

?

Cbr (2/2)

O668

fruit

Rosaceae - Malus domestica

26 (43)

~ 500-, 40 ad, 20 L4, 20 L3, 60 d, 200 L2, 100 L1

3

P2

Cbr (18/18)

O669

fruit

Rosaceae - Malus domestica

22 (37)

~ 1000+, 100 ad, 100 L4, 100 L3, 500 L2d, 500 L1,10 d

4

P2

Cbr (20/20)

O670

fruit

Rosaceae - Malus domestica

36 (62)

1

1

?

Cbr (1/1)

S101

stem

Apiaceae - Heracleum sphondylium

6

~ 30, dauers, 1 L3, all stages

2

P

Cel (6/6)

S102

stem

Asteraceae

4

~ 30, non-dauer larvae

2

P1

Cel (6/6)

S103

stem

Asteraceae - Cirsium oleraceum?

8

~ 2000 total, proportions ca. 30 ad, 200 d, 100 L2d, 100 L1

4

P3

Cel (6/6)

S104

stem

Asteraceae - Petasites sp.

1

2 dauers (L4 next day)

1

D

Cel (3/3)

S105

stem

Asteraceae - Cirsium oleraceum?

5

~ 1000 total, proportions ca. 5 ad, 10 L4, 5 L3, 50 L2d, 50 L1

3

P2

Cel (6/6)

S106

stem

Apiaceae - Heracleum sphondylium

1

~ 500 total, proportions ca. 30 ad, 15 L4, 10 L3, 30 L2, 30 L1

3

P1

Cel (6/6)

S107

stem

Asteraceae - Petasites sp.

4

6, stage unclear

1

?

Cel (6/6)

S108

3 snails

3 snails

-

0

-

-

S109

stem

Asteraceae - Cirsium oleraceum?

2

few, at least 1 dauer, next day 1 L2

1

?

Cel (5/5)

S110

stem

Asteraceae - Petasites sp.

6

~ 50, 5 ad, 5 L4, 5 L3, 5 d ?

2

P

Cel (6/6)


Scoring of Caenorhabditis populations in ten Orsay apples (O661-670) and ten Santeuil samples (S101-S110). These sets were chosen to represent a variety of Caenorhabditis population size and type, and maximize the proportion of positive samples (that is, the presence of Caenorhabditis is less frequent than would be suggested by these sets; see Additional Files 1 and 2 and Figure 5). Only a part of each apple was analyzed, which is taken into account for in the total census. Weight is in grams. Total census is on a Log scale. d: dauer larva. ad: adult. The tentative stage of the population is indicated: D, dauer stage; P, proliferating; P1, early stage of proliferation (no dauers); P2, intermediate stage (L3 and L4 stages present, as well as L2d or dauers); P3, late stage (many L1, L2d, dauers). The Caenorhabditis species was identified for a set of individuals of each population, as indicated in the last column. ~, approximately; Cbr: C. briggsae; Cel: C. elegans.

FĂ©lix and Duveau BMC Biology 2012 10:59   doi:10.1186/1741-7007-10-59

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