Est-6 expression in antennae. (A) Quantitative PCR (qPCR) analysis of Est-6 expression on cDNAs from wild-type (CS) male and female antennae, male gustatory appendages and heads without sensory appendages. Est-6 expression level was normalized to that of pgk. Data were obtained from triplicate biological experiments and are given as the mean ± SEM. (B) Third antennal segment, posterior view of left antenna. Medial is to the left. (C) Expression of Est-6 in Est-6Gal4/UAS-mCD8-GFP male. (D) Anti-ELAV staining of Est-6Gal4/UAS-mCD8-GFP antennae. (E) Merge of (C, D). (F) higher magnification of (E). Scale bars = 20 μM (except for (F), scale bar = 8 μm).
Chertemps et al. BMC Biology 2012 10:56 doi:10.1186/1741-7007-10-56