The translatome of mistranslating cells. A) Graphical representation of the correlation between the profiles of total mRNA (transcriptome) and polysome-associated (translatome) mRNA. The y-axis represents M values of the ratio of total mRNA transcript fractions at time-points T90' and T0'. The x-axis represents M values of the ratio of polysome-associated transcripts at time-points T90' and T0'. B) Cross stress comparison of translatome DEGs using GO terms enrichment analysis . Mistranslation translatome DEGs (T90') were used for this comparative analysis. The translatome data of cells exposed to environmental stress were obtained from Halbeisen et al. . C) Schematic representation of genes shown in the panel-A quadrants, which have higher variation of fold values between the transcriptome and translatome. The data show that gene expression deregulation was independent of the CUG content and that ribosomal proteins are not directly affected by Ser misincorporation at CUGs.
Paredes et al. BMC Biology 2012 10:55 doi:10.1186/1741-7007-10-55