Open Access Research article

Low level genome mistranslations deregulate the transcriptome and translatome and generate proteotoxic stress in yeast

João A Paredes1, Laura Carreto1, João Simões1, Ana R Bezerra1, Ana C Gomes1, Rodrigo Santamaria2, Misha Kapushesky2, Gabriela R Moura1 and Manuel AS Santos1*

Author Affiliations

1 RNA Biology Laboratory, Department of Biology and CESAM, University of Aveiro, 3810-193 Aveiro, Portugal

2 European Bioinformatics Institute, Wellcome Trust Genome Campus, Hinxton, CB10 1SD Cambridge, UK

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BMC Biology 2012, 10:55  doi:10.1186/1741-7007-10-55

Published: 20 June 2012

Additional files

Additional file 1:

Figure S1. Growth curves of Control and tetO-tRNA clones when tRNACAGSer is induced at OD600 = 0.1 (for further information see legend in Additional file 15).

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Additional file 2:

Figure S2. Effect of mistranslation induction in yeast viability and re-grow in new medium (for further information see legend in Additional file 15).

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Additional file 3:

Figure S3. Global yeast transcriptional responses to mRNA mistranslations and environmental stressors (for further information see legend in Additional file 15).

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Additional file 4:

Table S1. DEGs found for each time point (for further information see legend below the table).

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Additional file 5:

Table S2. Gene overlap between ESR and mistranslation DEGs for each time point (for further information see legend below the table).

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Additional file 6:

Figure S4. Transcription profiles of the yeast core stress response (for further information see legend in Additional file 15).

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Additional file 7:

Table S3. Series of tables representing gene expression response to mistranslation obtained in this study and all the others datasets discussed and compared along the publication.

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Additional file 8:

Figure S5. Transcriptome profiles highlighting yeast chaperone and protein folding genes involved in the stress response (for further information see legend in Additional file 15).

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Additional file 9:

Figure S6. Mistranslations and environmental stressors and their negative impact on the translational machinery (for further information see legend in Additional file 15).

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Additional file 10:

Figure S7. Comparison of the translatome profiles of mistranslating cells at T90' with the translatome profiles of cells exposed to environmental stressors (for further information see legend in Additional file 15).

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Additional file 11:

Figure S8. Mistranslation and environmental stressors and their impact in the unfolded protein response related genes (for further information see legend in Additional file 15).

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Additional file 12:

Figure S9. Promoter elements that regulate the stress response induced by mistranslations (for further information see legend in Additional file 15).

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Additional file 13:

Figure S10. Mistranslation and environmental stressors and their impact in the ubiquitin-proteasome pathway related genes (for further information see legend in Additional file 15).

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Additional file 14:

Figure S11. Mistranslations affect stress and ribosome linked chaperone networks in a time dependent manner (for further information see legend in Additional file 15).

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Additional file 15:

Legends for all supplementary figures (Figures S1 to S11).

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