Localization of PfCK2 subunits over the life cycle of the blood-stage parasite. (A-C) Hemagglutinin (HA)-tagged PfCK2 subunits (green) localized to both nuclear and cytoplasmic compartments in rings, trophozoites and schizonts. The limits of the parasitophorous vacuole membrane (PVM) are marked by Exp-2 (red). (D) HA-tagged PfCK2 subunits were detected in both the cytoplasmic and nuclear fraction. Western-blot analysis was performed on the cytoplasmic (C) and nuclear (N) fraction of unsynchronized PfCK2α, PfCK2β1, and PfCK2β2 parasites using anti-HA antibody, which gave bands in both the fractions. Predicted molecular weights of the proteins are: PfCK2α, 39.8 kDa; PfCK2β1, 28.3 kDa; PfCK2β2, 45.2 kDa. (E) The same C and N fractions were probed with antibodies against aldolase, a cytoplasmic marker, and histone H3, a nuclear marker, to assess the purity of the fractions. Aldolase gave a band at 40 kDa in the cytoplasmic fractions only, whereas the histone H3 band at 17 kDa was seen only in the nuclear fractions.
Dastidar et al. BMC Biology 2012 10:5 doi:10.1186/1741-7007-10-5