Open Access Highly Accessed Research article

Macondo crude oil from the Deepwater Horizon oil spill disrupts specific developmental processes during zebrafish embryogenesis

T Yvanka de Soysa1, Allison Ulrich1, Timo Friedrich2, Danielle Pite1, Shannon L Compton1, Deborah Ok1, Rebecca L Bernardos1, Gerald B Downes2, Shizuka Hsieh1, Rachael Stein1, M Caterina Lagdameo1, Katherine Halvorsen1, Lydia-Rose Kesich1 and Michael JF Barresi1*

Author Affiliations

1 Biological Sciences, Smith College, Northampton, MA 01063, USA

2 Molecular and Cellular Biology, University of Massachusetts, Amherst MA, USA

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BMC Biology 2012, 10:40  doi:10.1186/1741-7007-10-40

Published: 4 May 2012

Additional files

Additional file 1:

Initial assessment of water accumulated fractions. This file provides greater explanation of the methods and results associated with our WAF mixing procedure, as well as initial assessments of the effects of different WAF concentrations on the development of zebrafish gross morphology.

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Additional file 2:

Expanded WAF chemical analysis. This file provides a detailed list of all the compounds and concentrations discovered following gas chromatography mass spectrometry analysis of our WAF. Supplemental Table S1: GCMS parameters for Agilent 7890A GC/5975C MSD. Supplemental Table S2: Aqueous solubilities and Henry's constants for selected hydrocarbons. Supplemental Table S3: Most prominent GCMS peaks in WAF. Supplemental Figure S1: Relative detection efficiencies of the SPME/GCMS method for selected aromatics and alkanes. Supplemental Figure S2: Total ion chromatogram. Supplemental Figure S3: Total ion chromatograms for the aqueous portion of a WAF sample and for a sample that includes some of the oily layer.

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