Additional file 11.

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Movie 11 - Sequential dye labeling demonstrates temporally distinct endocytic zones. This time-lapse movie shows that endocytic hot-spots at two separate time-points occur at spatially distinct areas of the same growth cone. The dual FM dye labelling assay was performed as described in Figure 8A. Briefly, a focal pulse of FM 2-10 (green) was applied at 00:00. A second micropipette then applied a subsequent focal pulse of FM 5-95 at 00:38. Movie 11 corresponds to Figure 8B-B''. The time (minutes:seconds) is indicated at the top left. Scale bar, 5 μm. Format: MOV (MPEG4 compression).

Format: MOV Size: 9.6MB Download file

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Hines et al. BMC Biology 2012 10:4   doi:10.1186/1741-7007-10-4