Additional file 2.

Smad2 is not phosphorylated by BMP2 stimulation, mechanical loading or a combination of both. hFOBs were seeded on collagen scaffolds and subjected to BMP2 stimulation, mechanical loading or a combination of both for indicated time points. As positive control, hFOBs were stimulated for 30 minutes with 100 pM TGF-β1. Protein lysates were analysed by western blot using specific antibodies.

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Kopf et al. BMC Biology 2012 10:37   doi:10.1186/1741-7007-10-37