Additional file 7.
Immunostaining of tumor spheroid (TS)-embryoid body (EB) at the end of a confrontation culture assay (60 h). Hematoxylin and eosin (H&E) staining (upper panel) shows the two tissues and their close association. CD34 immunoperoxidase staining (lower panel) identifies endothelial differentiation within the EB and the extension of the pseudovascular structures into the TS (arrows). Scale bars: 200 μm (left) and 100 μm (right) images.
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Vinci et al. BMC Biology 2012 10:29 doi:10.1186/1741-7007-10-29