Additional file 12.

PI-103 and 17-N-allylamino-17-demethoxygeldanamycin (17-AAG) concentration-dependent inhibition of tumor spheroid growth kinetics: fully automated imaging and analysis. (a) KNS42 and (b) LICR-LON-HN4 spheroids were treated on day 4 as described above, with PI-103 (0 to 2 μM) and 17-AAG (0 to 2.93 μM) respectively, accordingly to their three-dimensional concentration inhibiting cell viability by 50% (GI50) values (see Table 2). A Celigo cytometer was used for automated imaging and analysis. Graphs show concentration-dependent inhibition of spheroid growth from day 4 to day 14. Values are means ± SD, n = 6; one representative of three separate experiments is shown.

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Vinci et al. BMC Biology 2012 10:29   doi:10.1186/1741-7007-10-29