Additional file 10.
Comparison of sensitivity to compounds in two-dimensional vs three-dimensional cultures. (a, b) MDA-MB-231 P cells were grown in two dimensions (monolayer) and in three dimensions (spheroids). Then, 4 days later, cells were treated with (a) 17-N-allylamino-17-demethoxygeldanamycin (17-AAG) (final concentrations 0 to 25 μM) or (b) CCT130234 (final concentrations 0 to 100 μM). Then, 72 h later, a CellTiter Glo luminescence assay for cell viability was performed and concentration inhibiting cell viability by 50% (GI50) values were determined. Values are means ± SD, n = 6; (a) and (b) are representative of three separate experiments.
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Vinci et al. BMC Biology 2012 10:29 doi:10.1186/1741-7007-10-29