Additional file 3.

Sensitivity of used YFP towards pH. The figure shows the normalized YFP fluorescence intensity at increasing pH values. Purified YFP was adjusted to an absorption of 0.05. The effect of the pH value on YFP fluorescence was measured in citric acid/disodium phosphate buffer with pH values ranging form 4.0 to 7.0. The pKa value is marked by doted lines.

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Potzkei et al. BMC Biology 2012 10:28   doi:10.1186/1741-7007-10-28