Validation of phasic and tonic currents recorded from CA1 pyramidal neurons at 0 mV holding potential as GABAergic currents. (A) Voltage-clamp recording showing the elimination of both GABAergic IPSCs and GABAergic tonic current in the presence of 100 μM picrotoxin in ACSF containing 30 μM Mg2+. (B) Holding current plotted at 1 s intervals during control condition (black), SNAP-5114 application (red), washout (blue) and picrotoxin application (brown) in the same experiment.
Héja et al. BMC Biology 2012 10:26 doi:10.1186/1741-7007-10-26