Table 2

Mnemiopsis homologs to ciliary and rhabodmeric phototransduction cascade components and their reciprocal best BLAST hit.

Protein name (GenBank accession number of query protein)

GenBank accession number of top

Mnemiopsis result (ML identifier)

E-value

Reciprocal best BLAST query result

Protein name [Species; GenBank accession number]

E-value

RNA-seq

Evidence?


Ciliary components


Opsin (ACB05673)

JQ724646

(ML13055)

2e-22

Opsin-3-like [Oreochromis niloticus; XP_003441288]

6e-31

Yes

G-alpha-s subunit (BAA81697)

JX564543

(ML012011)

1e-93

guanine nucleotide binding protein, alpha stimulating activity polypeptide [Daphnia pulex; EFX88427.1]

3e-177

Yes

G-alpha-i subunit (ACB05685.1)

JQ724654

(ML156514)

2e-129

G protein alpha subunit i class [Halocynthia roretzi; BAB79197.1]

2e-177

Yes

Transducin G-alpha-t1 (AAB01735_1)

JX564546

(MLRB156557)

9e-73

guanine nucleotide-binding protein G(i) subunit alpha-2 [Mus musculus; NP_032164.2]

3e-173

Yes

Transducin G-gamma-t1 (AAH25929_1)

JX564547

(ML17031)

7e-07

guanine nucleotide-binding protein G(T) subunit gamma-T1 [Otolemur garnettii; XP_003782721]

1e-06

Yes

GRK1 G protein-coupled receptor kinase 1 (AAH96611_1)

JX564550

(MLRB009169)

9e-110

G protein-coupled receptor kinase 5 [Callithrix jacchus; XP_002756686]

0.0

No

GMP-PDE alpha rod (NP_666198_1); GMP-PDE beta rod (P23440_3)

JX564548

(MLRB03248)

1e-97;

5e-99

sperm phosphodiesterase 5-like [Saccoglossus kowalevskii; XP_002733933]

0.0

Yes

GMP-PDE delta (O55057_1)

JX564549

(ML233327a)

6e-55

cGMP-specific rod phosphodiesterase 6D delta [Trichoplax adhaerens; XP_002113713]

8e-55

Yes

Phosphodiesterase (ACB05690)

JQ724657

(ML096829)

4e-105

High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A-like [Danio rerio; XP_692819.2]

1e-163

Yes

Cyclic nucleotide gated ion channel (CAB42891.1)

JX564544

(ML08605)

and

JX564545

(ML054419)

2e-105

1e-115

cGMP-gated cation channel alpha-1-like, partial [Cricetulus griseus; XP_003515326]

predicted protein [Nematostella vectensis; XP_001641603.1]

2e-124

1e-121

Yes

RGS9-1 regulator of G-protein signaling 9 isoform 1 (NP_035398_2)

JX564552

(MLRB369320)

2e-14

regulator of G-protein signaling loco-like [Megachile rotundata; XP_003705980]

1e-27

No

GC1 guanylyl cyclase GC-E precursor (NP_032218_2)

JX564553

(ML17474)

2e-131

natriuretic peptide receptor 1-like [Saccoglossus kowalevskii; XP_002734106]

0.0

Yes

Recoverin (NP_033064_1);

GCAP1 guanylyl cyclase-activating protein 1 (NP_032215_2);

GCAP2 guanylyl cyclase-activating protein 2 (NP_666191_1)

JX564551

(ML096819)

9e-51;

1e-32;

2e-38

Hypothetical protein DAPPUDRAFT_65663 [Daphnia pulex; EFX65172]

2e-121

Yes


Rhabdomeric components


G-alpha-q subunit (ACB05683)

JQ724653

(ML009153)

4e-105

Heterotrimeric GTP-binding protein alpha subunit G-alpha-q [Litopenaeus vannamei; AAT44837.1]

8e-134

Yes

Phospholipase C (ACB05675)

JQ724649

(ML04921)

1e-178

Predicted protein [Nematostella vectensis; XP_001635876.1]

≤1e-1000

Yes

Trp-C protein (ACB05689)

JQ724656

(ML234550)

1e-11

Similar to TRP gamma cation channel [Nasonia vitripennis; XP_001604587.1]

1e-53

Yes


Shared components


Visual G beta (ACB05681)

JQ724652

(ML02234)

7e-162

G-protein beta subunit [Meloidogyne javanica; ACB97665.1]

≤1e-1000

Yes

Rhodopsin kinase (ACB05677)

JQ724650

(ML04904)

8e-85

Beta-adrenergic receptor kinase 2 [Danio rerio; NP_001128197.1]

≤1e-1000

Yes

Arrestin (ACB05679 and P20443_1)

JQ724651

(ML047926)

4e-55

TRIADDRAFT_64255 [Trichoplax adhaerens; XP_002116188.1]

≤1e-1000

Yes

Retinal-binding protein (ACB05687)

JQ724655

(ML167044)

2e-28

Similar to SEC14-like protein 1 isoform 4 [Canis familiaris; XP_857362.1]

≤1e-1000

Yes


For each query protein, the corresponding Mnemiopsis protein model is listed with the E-value of the top BLAST result. The Mnemiopsis protein model was then used as a query in a reciprocal best BLAST search of the non-redundant protein database (NCBI) and the top result is listed along with the E-value of the BLAST result. RNA-seq data supports the developmental mRNA gene expression of each of these protein models. BLAST: Basic Local Alignment Search Tool; ML: Mnemiopsis leidyi; PDE: phosphodiesterase; TRP: transient receptor potential.

Schnitzler et al. BMC Biology 2012 10:107   doi:10.1186/1741-7007-10-107

Open Data