Table 1

Accuracy and precision of DNA quantitation by different methods

Species

Data source

Ratio of means

Standard deviation


(post-S/pre-S)

Pre-S

Post-S


Trypanosoma brucei

Flow cytometry

1.83

0.08

0.10

T. brucei

Manual (nucleus)

1.62

0.11

0.16

T. brucei

Auto (nucleus)

1.90

0.14

0.24

Leishmania mexicana

Flow cytometry

2.07

0.13

0.26

L. mexicana

Manual (nucleus)

1.85

0.26

0.41

L. mexicana

Auto (nucleus)

2.38

0.17

0.29


Histograms of total DNA content as determined by flow cytometry, nuclear DNA content as measured manually from 4', 6-diamidino-2-phenylindole (DAPI) images (manual) and nuclear DNA content as measured automatically from color-deconvolved images (auto) were fitted to a model of pre-S-phase, post-S-phase and during S-phase changes in DNA content through the cell cycle (Figure 5). The fitting parameters are summarized this table, all values are normalized to the measured pre-S-phase DNA for that data source. The standard deviation required to match the experimental error in the pre-/post-S-phase peaks is a measure of precision. The ratio of the mean of the post-S-phase to the pre-S-phase peak gives a measure of accuracy and is expected to be 2.

Wheeler et al. BMC Biology 2012 10:1   doi:10.1186/1741-7007-10-1

Open Data